artepillin c Search Results


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Chemical structure of <t>artepillin</t> <t>C</t> (3,5-diprenyl-4-hydroxycinnamic acid).
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Chemical structure of <t>artepillin</t> <t>C</t> (3,5-diprenyl-4-hydroxycinnamic acid).
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Image Search Results


Chemical structure of artepillin C (3,5-diprenyl-4-hydroxycinnamic acid).

Journal: International Journal of Oncology

Article Title: Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) sensitizes LNCaP prostate cancer cells to TRAIL-induced apoptosis

doi: 10.3892/ijo.2012.1527

Figure Lengend Snippet: Chemical structure of artepillin C (3,5-diprenyl-4-hydroxycinnamic acid).

Article Snippet: Artepillin C was provided by Wako Pure Chemicals (Osaka, Japan) as a natural component isolated from Brazilian green propolis.

Techniques:

Cytotoxic and apoptotic effects of TRAIL in combination with artepillin C in LNCaP prostate cancer cells. Cells were incubated for 24 h with 100 ng/ml TRAIL and/or 50–100 μ M artepillin C. (A) Cytotoxic activity of TRAIL in combination with artepillin C in LNCaP cells. The percentage of cell death was measured using the MTT cytotoxicity assay. The values represent the means ± SD of three independent experiments performed in quadruplicate, n=12 (p<0.0001 TRAIL + artepillin C compared to TRAIL or artepillin C alone as shown by ANOVA). (B) TRAIL-induced apoptosis in combination with artepillin C in LNCaP cells. Apoptotic cell death was detected by flow cytometry using annexin V-FITC staining. The values represent the means ± SD of three independent experiments performed in duplicate, n=6 (p<0.0001 TRAIL + artepillin C compared to TRAIL or artepillin C alone as shown by ANOVA). (C) TRAIL-induced apoptosis in combination with artepillin C in LNCaP cells: (a) control cells, (b) cells incubated with 100 ng/ml TRAIL, (c) cells incubated with 50 μ M artepillin C, (d) cells incubated with 100 ng/ml TRAIL and 50 μ M artepillin C, (e) cells incubated with 100 μ M arte pillin C and (f) cells incubated with 100 ng/ml TRAIL and 100 μ M artepillin C. Apoptotic cell death was detected and visualised by fluorescence microscopy using Annexin V-FITC staining. Healthy cells (stained with Hoechst 33342) emitted blue fluorescence and apoptotic cells (stained with Hoechst 33342 and annexin V-FITC) emitted green and blue fluorescence (indicated by arrows).

Journal: International Journal of Oncology

Article Title: Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) sensitizes LNCaP prostate cancer cells to TRAIL-induced apoptosis

doi: 10.3892/ijo.2012.1527

Figure Lengend Snippet: Cytotoxic and apoptotic effects of TRAIL in combination with artepillin C in LNCaP prostate cancer cells. Cells were incubated for 24 h with 100 ng/ml TRAIL and/or 50–100 μ M artepillin C. (A) Cytotoxic activity of TRAIL in combination with artepillin C in LNCaP cells. The percentage of cell death was measured using the MTT cytotoxicity assay. The values represent the means ± SD of three independent experiments performed in quadruplicate, n=12 (p<0.0001 TRAIL + artepillin C compared to TRAIL or artepillin C alone as shown by ANOVA). (B) TRAIL-induced apoptosis in combination with artepillin C in LNCaP cells. Apoptotic cell death was detected by flow cytometry using annexin V-FITC staining. The values represent the means ± SD of three independent experiments performed in duplicate, n=6 (p<0.0001 TRAIL + artepillin C compared to TRAIL or artepillin C alone as shown by ANOVA). (C) TRAIL-induced apoptosis in combination with artepillin C in LNCaP cells: (a) control cells, (b) cells incubated with 100 ng/ml TRAIL, (c) cells incubated with 50 μ M artepillin C, (d) cells incubated with 100 ng/ml TRAIL and 50 μ M artepillin C, (e) cells incubated with 100 μ M arte pillin C and (f) cells incubated with 100 ng/ml TRAIL and 100 μ M artepillin C. Apoptotic cell death was detected and visualised by fluorescence microscopy using Annexin V-FITC staining. Healthy cells (stained with Hoechst 33342) emitted blue fluorescence and apoptotic cells (stained with Hoechst 33342 and annexin V-FITC) emitted green and blue fluorescence (indicated by arrows).

Article Snippet: Artepillin C was provided by Wako Pure Chemicals (Osaka, Japan) as a natural component isolated from Brazilian green propolis.

Techniques: Incubation, Activity Assay, Cytotoxicity Assay, Flow Cytometry, Staining, Fluorescence, Microscopy

Effects of artepillin C on death receptor expression in LNCaP pro state cancer cells. Cells were incubated for 24 h with 50–100 μ M artepillin C. The surface expression of TRAIL-R1 and TRAIL-R2 on LNCaP cells was measured by flow cytometry. Representative histograms and the average mean fluorescence for (A) TRAIL-R1 expression and for (B) TRAIL-R2 expression are shown from three independent experiments performed in duplicate, n=6. The values represent the means ± SD (p<0.0001 artepillin C compared to control as shown by ANOVA).

Journal: International Journal of Oncology

Article Title: Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) sensitizes LNCaP prostate cancer cells to TRAIL-induced apoptosis

doi: 10.3892/ijo.2012.1527

Figure Lengend Snippet: Effects of artepillin C on death receptor expression in LNCaP pro state cancer cells. Cells were incubated for 24 h with 50–100 μ M artepillin C. The surface expression of TRAIL-R1 and TRAIL-R2 on LNCaP cells was measured by flow cytometry. Representative histograms and the average mean fluorescence for (A) TRAIL-R1 expression and for (B) TRAIL-R2 expression are shown from three independent experiments performed in duplicate, n=6. The values represent the means ± SD (p<0.0001 artepillin C compared to control as shown by ANOVA).

Article Snippet: Artepillin C was provided by Wako Pure Chemicals (Osaka, Japan) as a natural component isolated from Brazilian green propolis.

Techniques: Expressing, Incubation, Flow Cytometry, Fluorescence

Effects of TRAIL in combination with artepillin C on caspase activities in LNCaP prostate cancer cells. Assessment of intracellular (A) caspase-8 and (B) caspase-3 activity in cells treated with 100 ng/ml TRAIL and/or 50–100 μ M artepillin C for 24 h. Caspase activities were measured using colorimetric protease assays based on the spectrophotometric detection of the chromophore, p -nitroaniline (pNa), after its cleavage from the labelled caspase substrates. The values represent the means ± SD of three independent experiments performed in duplicate, n=6 (p<0.0001 TRAIL + artepillin C compared to TRAIL or artepillin C alone as shown by ANOVA).

Journal: International Journal of Oncology

Article Title: Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) sensitizes LNCaP prostate cancer cells to TRAIL-induced apoptosis

doi: 10.3892/ijo.2012.1527

Figure Lengend Snippet: Effects of TRAIL in combination with artepillin C on caspase activities in LNCaP prostate cancer cells. Assessment of intracellular (A) caspase-8 and (B) caspase-3 activity in cells treated with 100 ng/ml TRAIL and/or 50–100 μ M artepillin C for 24 h. Caspase activities were measured using colorimetric protease assays based on the spectrophotometric detection of the chromophore, p -nitroaniline (pNa), after its cleavage from the labelled caspase substrates. The values represent the means ± SD of three independent experiments performed in duplicate, n=6 (p<0.0001 TRAIL + artepillin C compared to TRAIL or artepillin C alone as shown by ANOVA).

Article Snippet: Artepillin C was provided by Wako Pure Chemicals (Osaka, Japan) as a natural component isolated from Brazilian green propolis.

Techniques: Activity Assay

Effects of TRAIL in combination with artepillin C on the mitochondrial membrane potential (ΔΨm) in LNCaP prostate cancer cells. Cells were incubated for 24 h with 100 ng/ml TRAIL and/or 50–100 μ M artepillin C. The values represent the meana ± SD of three independent experiments performed in duplicate, n=6 (p<0.001 TRAIL + artepillin C compared to TRAIL or artepillin C alone as shown by ANOVA). (A) TRAIL in combination with artepillin C induced a loss of ΔΨm in LNCaP cells. (B) Disruption of ΔΨm in LNCaP cells was assessed by fluorescent microscopic analysis of DePsipher staining using the following treatment conditions: (a) control cells, (b) cells incubated with 100 ng/ml TRAIL, (c) cells incubated with 50 μ M artepillin C, (d) cells incubated with 100 ng/ml TRAIL and 50 μ M artepillin C, (e) incubated with 100 μ M artepillin C and (f) cells incubated with 100 ng/ml TRAIL and 100 μ M artepillin C. Red fluo rescence is emitted from aggregates of DePsipher, which are formed within the mitochondria of healthy cells. Green fluorescence reveals the monomeric form of the DePsipher molecule, which appears in the cytosol after mitochondrial membrane depolarisation (indicated by arrows).

Journal: International Journal of Oncology

Article Title: Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) sensitizes LNCaP prostate cancer cells to TRAIL-induced apoptosis

doi: 10.3892/ijo.2012.1527

Figure Lengend Snippet: Effects of TRAIL in combination with artepillin C on the mitochondrial membrane potential (ΔΨm) in LNCaP prostate cancer cells. Cells were incubated for 24 h with 100 ng/ml TRAIL and/or 50–100 μ M artepillin C. The values represent the meana ± SD of three independent experiments performed in duplicate, n=6 (p<0.001 TRAIL + artepillin C compared to TRAIL or artepillin C alone as shown by ANOVA). (A) TRAIL in combination with artepillin C induced a loss of ΔΨm in LNCaP cells. (B) Disruption of ΔΨm in LNCaP cells was assessed by fluorescent microscopic analysis of DePsipher staining using the following treatment conditions: (a) control cells, (b) cells incubated with 100 ng/ml TRAIL, (c) cells incubated with 50 μ M artepillin C, (d) cells incubated with 100 ng/ml TRAIL and 50 μ M artepillin C, (e) incubated with 100 μ M artepillin C and (f) cells incubated with 100 ng/ml TRAIL and 100 μ M artepillin C. Red fluo rescence is emitted from aggregates of DePsipher, which are formed within the mitochondria of healthy cells. Green fluorescence reveals the monomeric form of the DePsipher molecule, which appears in the cytosol after mitochondrial membrane depolarisation (indicated by arrows).

Article Snippet: Artepillin C was provided by Wako Pure Chemicals (Osaka, Japan) as a natural component isolated from Brazilian green propolis.

Techniques: Incubation, Staining, Fluorescence

Effects of artepillin C and TRAIL on NF-κB activity in LNCaP prostate cancer cells. Cells were incubated for 24 h with 50–100 μ M artepillin C and/or 100 ng/ml TRAIL. The values represent the means ± SD of three independent experiments performed in duplicate, n=6 (p<0.0001 TRAIL + artepillin C compared to TRAIL or artepillin C alone as shown by ANOVA). The effects of artepillin C and/or TRAIL on the NF-κB (p65) binding activity in LNCaP nuclear extracts were measured using the ELISA-based TransAM NF-κB assay.

Journal: International Journal of Oncology

Article Title: Artepillin C (3,5-diprenyl-4-hydroxycinnamic acid) sensitizes LNCaP prostate cancer cells to TRAIL-induced apoptosis

doi: 10.3892/ijo.2012.1527

Figure Lengend Snippet: Effects of artepillin C and TRAIL on NF-κB activity in LNCaP prostate cancer cells. Cells were incubated for 24 h with 50–100 μ M artepillin C and/or 100 ng/ml TRAIL. The values represent the means ± SD of three independent experiments performed in duplicate, n=6 (p<0.0001 TRAIL + artepillin C compared to TRAIL or artepillin C alone as shown by ANOVA). The effects of artepillin C and/or TRAIL on the NF-κB (p65) binding activity in LNCaP nuclear extracts were measured using the ELISA-based TransAM NF-κB assay.

Article Snippet: Artepillin C was provided by Wako Pure Chemicals (Osaka, Japan) as a natural component isolated from Brazilian green propolis.

Techniques: Activity Assay, Incubation, Binding Assay, Enzyme-linked Immunosorbent Assay